It was located in the village of Novo-Blagoveshchenie, which in 1777 became part of the city of Borisoglebsk. The church belonged to the Yaroslavl Spaso-Preobrazhensky Monastery until 1764.
There is a hypothesis that it was built by Moscow builders. This is probably due to the design features of the Annunciation Church. Indeed, the Moscow cult architecture of the XVII century is characterized by small pillarless temples decorated with kokoshnikov slides. The Annunciation Church was built in this style. It is the only pillar-less temple in the city. The temple is based on a square of 11 by 11 m, the wall thickness is 1m 30cm.
The main shrine of the church was the Don Icon of the Blessed Virgin Mary. In 1771, she saved the inhabitants of the settlement from a pestilence. The memory of the veneration of the miraculous image is preserved in the name of Donskaya Street. Today, the Miraculous Donskoy image of the Mother of God is located in the Resurrection Cathedral.
The official closing date of the Annunciation Church is November 21, 1929. The premises were transferred for use for the cultural needs of public organizations. One of the most long-suffering churches in the Soviet period turned out to be the Church of the Annunciation. Its premises housed the Stalin Club, then a bakery. They tried to disassemble the church into bricks, but the brickwork turned out to be too strong.
In 2000, the parish was registered, and on the patronal feast of St. Alexy – February 25, 2001, the first Liturgy was held.
Old icons are returning to the temple. They are brought by parishioners and guests of the temple. The icons of the Kazan Mother of God, Peter and Paul of 1885, St. Nicholas, depicted in full growth, and many others returned.